Endophytic fungal diversity in Theobroma cacao (cacao) and T. grandiflorum (cupuaçu) trees and their potential for growth promotion and biocontrol of black-pod disease.
Identifieur interne : 000242 ( Main/Exploration ); précédent : 000241; suivant : 000243Endophytic fungal diversity in Theobroma cacao (cacao) and T. grandiflorum (cupuaçu) trees and their potential for growth promotion and biocontrol of black-pod disease.
Auteurs : Rogério Eiji Hanada [Brésil] ; Alan William V. Pomella ; Heron Salazar Costa ; José Luiz Bezerra ; Leandro L. Loguercio ; José O. PereiraSource :
- Fungal biology [ 1878-6146 ]
Descripteurs français
- KwdFr :
- Antibiose (MeSH), Arbres (microbiologie), Arbres (parasitologie), Biodiversité (MeSH), Cacaoyer (microbiologie), Cacaoyer (parasitologie), Champignons (génétique), Champignons (isolement et purification), Champignons (physiologie), Lutte biologique contre les nuisibles (méthodes), Maladies des plantes (parasitologie), Phytophthora (physiologie), Symbiose (MeSH).
- MESH :
- génétique : Champignons.
- isolement et purification : Champignons.
- microbiologie : Arbres, Cacaoyer.
- méthodes : Lutte biologique contre les nuisibles.
- parasitologie : Arbres, Cacaoyer, Maladies des plantes.
- physiologie : Champignons, Phytophthora.
- Antibiose, Biodiversité, Symbiose.
English descriptors
- KwdEn :
- Antibiosis (MeSH), Biodiversity (MeSH), Cacao (microbiology), Cacao (parasitology), Fungi (genetics), Fungi (isolation & purification), Fungi (physiology), Pest Control, Biological (methods), Phytophthora (physiology), Plant Diseases (parasitology), Symbiosis (MeSH), Trees (microbiology), Trees (parasitology).
- MESH :
- genetics : Fungi.
- isolation & purification : Fungi.
- methods : Pest Control, Biological.
- microbiology : Cacao, Trees.
- parasitology : Cacao, Plant Diseases, Trees.
- physiology : Fungi, Phytophthora.
- Antibiosis, Biodiversity, Symbiosis.
Abstract
The endophytic niches of plants are a rich source of microbes that can directly and indirectly promote plant protection, growth and development. The diversity of culturable endophytic fungi from stems and branches of Theobroma cacao (cacao) and Theobroma grandiflorum (cupuaçu) trees growing in the Amazon region of Brazil was assessed. The collection of fungal endophytic isolates obtained was applied in field experiments to evaluate their potential as biocontrol agents against Phytophthora palmivora, the causal agent of the black-pod rot disease of cacao, one of the most important pathogens in cocoa-producing regions worldwide. The isolated endophytic fungi from 60 traditional, farmer-planted, healthy cacao and 10 cupuaçu plants were cultured in PDA under conditions inducing sporulation. Isolates were classified based upon the morphological characteristics of their cultures and reproductive structures. Spore suspensions from a total of 103 isolates that could be classified at least up to genus level were tested against P. palmivora in pods attached to cacao trees in the field. Results indicated that ∼70% of isolates showed biocontrol effects to a certain extent, suggesting that culturable endophytic fungal biodiversity in this system is of a mostly mutualistic type of interaction with the host. Eight isolates from genera Trichoderma (reference isolate), Pestalotiopsis, Curvularia, Tolypocladium and Fusarium showed the highest level of activity against the pathogen, and were further characterized. All demonstrated their endophytic nature by colonizing axenic cacao plantlets, and confirmed their biocontrol activity on attached pods trials by showing significant decrease in disease severity in relation to the positive control. None, however, showed detectable growth-promotion effects. Aspects related to endophytic biodiversity and host-pathogen-endophyte interactions in the environment of this study were discussed on the context of developing sustainable strategies for biological control of black-pod rot of cacao.
DOI: 10.1016/j.funbio.2010.08.006
PubMed: 21036333
Affiliations:
Links toward previous steps (curation, corpus...)
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Postal 478, 69083-000 Manaus-AM</wicri:regionArea><placeName><region type="state">Amazonas (Brésil)</region></placeName></affiliation></author><author><name sortKey="Pomella, Alan William V" sort="Pomella, Alan William V" uniqKey="Pomella A" first="Alan William V" last="Pomella">Alan William V. Pomella</name></author><author><name sortKey="Costa, Heron Salazar" sort="Costa, Heron Salazar" uniqKey="Costa H" first="Heron Salazar" last="Costa">Heron Salazar Costa</name></author><author><name sortKey="Bezerra, Jose Luiz" sort="Bezerra, Jose Luiz" uniqKey="Bezerra J" first="José Luiz" last="Bezerra">José Luiz Bezerra</name></author><author><name sortKey="Loguercio, Leandro L" sort="Loguercio, Leandro L" uniqKey="Loguercio L" first="Leandro L" last="Loguercio">Leandro L. Loguercio</name></author><author><name sortKey="Pereira, Jose O" sort="Pereira, Jose O" uniqKey="Pereira J" first="José O" last="Pereira">José O. Pereira</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2010">2010 Nov-Dec</date><idno type="RBID">pubmed:21036333</idno><idno type="pmid">21036333</idno><idno type="doi">10.1016/j.funbio.2010.08.006</idno><idno type="wicri:Area/Main/Corpus">000241</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">000241</idno><idno type="wicri:Area/Main/Curation">000241</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Curation">000241</idno><idno type="wicri:Area/Main/Exploration">000241</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Endophytic fungal diversity in Theobroma cacao (cacao) and T. grandiflorum (cupuaçu) trees and their potential for growth promotion and biocontrol of black-pod disease.</title><author><name sortKey="Hanada, Rogerio Eiji" sort="Hanada, Rogerio Eiji" uniqKey="Hanada R" first="Rogério Eiji" last="Hanada">Rogério Eiji Hanada</name><affiliation wicri:level="2"><nlm:affiliation>Instituto Nacional de Pesquisas da Amazônia (INPA/CPPF), Av. André Araujo, 2936, Petrópolis, Cx. Postal 478, 69083-000 Manaus-AM, Brazil.</nlm:affiliation><country xml:lang="fr">Brésil</country><wicri:regionArea>Instituto Nacional de Pesquisas da Amazônia (INPA/CPPF), Av. André Araujo, 2936, Petrópolis, Cx. Postal 478, 69083-000 Manaus-AM</wicri:regionArea><placeName><region type="state">Amazonas (Brésil)</region></placeName></affiliation></author><author><name sortKey="Pomella, Alan William V" sort="Pomella, Alan William V" uniqKey="Pomella A" first="Alan William V" last="Pomella">Alan William V. Pomella</name></author><author><name sortKey="Costa, Heron Salazar" sort="Costa, Heron Salazar" uniqKey="Costa H" first="Heron Salazar" last="Costa">Heron Salazar Costa</name></author><author><name sortKey="Bezerra, Jose Luiz" sort="Bezerra, Jose Luiz" uniqKey="Bezerra J" first="José Luiz" last="Bezerra">José Luiz Bezerra</name></author><author><name sortKey="Loguercio, Leandro L" sort="Loguercio, Leandro L" uniqKey="Loguercio L" first="Leandro L" last="Loguercio">Leandro L. Loguercio</name></author><author><name sortKey="Pereira, Jose O" sort="Pereira, Jose O" uniqKey="Pereira J" first="José O" last="Pereira">José O. Pereira</name></author></analytic><series><title level="j">Fungal biology</title><idno type="ISSN">1878-6146</idno></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Antibiosis (MeSH)</term><term>Biodiversity (MeSH)</term><term>Cacao (microbiology)</term><term>Cacao (parasitology)</term><term>Fungi (genetics)</term><term>Fungi (isolation & purification)</term><term>Fungi (physiology)</term><term>Pest Control, Biological (methods)</term><term>Phytophthora (physiology)</term><term>Plant Diseases (parasitology)</term><term>Symbiosis (MeSH)</term><term>Trees (microbiology)</term><term>Trees (parasitology)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Antibiose (MeSH)</term><term>Arbres (microbiologie)</term><term>Arbres (parasitologie)</term><term>Biodiversité (MeSH)</term><term>Cacaoyer (microbiologie)</term><term>Cacaoyer (parasitologie)</term><term>Champignons (génétique)</term><term>Champignons (isolement et purification)</term><term>Champignons (physiologie)</term><term>Lutte biologique contre les nuisibles (méthodes)</term><term>Maladies des plantes (parasitologie)</term><term>Phytophthora (physiologie)</term><term>Symbiose (MeSH)</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Fungi</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Champignons</term></keywords><keywords scheme="MESH" qualifier="isolation & purification" xml:lang="en"><term>Fungi</term></keywords><keywords scheme="MESH" qualifier="isolement et purification" xml:lang="fr"><term>Champignons</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Pest Control, Biological</term></keywords><keywords scheme="MESH" qualifier="microbiologie" xml:lang="fr"><term>Arbres</term><term>Cacaoyer</term></keywords><keywords scheme="MESH" qualifier="microbiology" xml:lang="en"><term>Cacao</term><term>Trees</term></keywords><keywords scheme="MESH" qualifier="méthodes" xml:lang="fr"><term>Lutte biologique contre les nuisibles</term></keywords><keywords scheme="MESH" qualifier="parasitologie" xml:lang="fr"><term>Arbres</term><term>Cacaoyer</term><term>Maladies des plantes</term></keywords><keywords scheme="MESH" qualifier="parasitology" xml:lang="en"><term>Cacao</term><term>Plant Diseases</term><term>Trees</term></keywords><keywords scheme="MESH" qualifier="physiologie" xml:lang="fr"><term>Champignons</term><term>Phytophthora</term></keywords><keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Fungi</term><term>Phytophthora</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Antibiosis</term><term>Biodiversity</term><term>Symbiosis</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Antibiose</term><term>Biodiversité</term><term>Symbiose</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The endophytic niches of plants are a rich source of microbes that can directly and indirectly promote plant protection, growth and development. The diversity of culturable endophytic fungi from stems and branches of Theobroma cacao (cacao) and Theobroma grandiflorum (cupuaçu) trees growing in the Amazon region of Brazil was assessed. The collection of fungal endophytic isolates obtained was applied in field experiments to evaluate their potential as biocontrol agents against Phytophthora palmivora, the causal agent of the black-pod rot disease of cacao, one of the most important pathogens in cocoa-producing regions worldwide. The isolated endophytic fungi from 60 traditional, farmer-planted, healthy cacao and 10 cupuaçu plants were cultured in PDA under conditions inducing sporulation. Isolates were classified based upon the morphological characteristics of their cultures and reproductive structures. Spore suspensions from a total of 103 isolates that could be classified at least up to genus level were tested against P. palmivora in pods attached to cacao trees in the field. Results indicated that ∼70% of isolates showed biocontrol effects to a certain extent, suggesting that culturable endophytic fungal biodiversity in this system is of a mostly mutualistic type of interaction with the host. Eight isolates from genera Trichoderma (reference isolate), Pestalotiopsis, Curvularia, Tolypocladium and Fusarium showed the highest level of activity against the pathogen, and were further characterized. All demonstrated their endophytic nature by colonizing axenic cacao plantlets, and confirmed their biocontrol activity on attached pods trials by showing significant decrease in disease severity in relation to the positive control. None, however, showed detectable growth-promotion effects. Aspects related to endophytic biodiversity and host-pathogen-endophyte interactions in the environment of this study were discussed on the context of developing sustainable strategies for biological control of black-pod rot of cacao.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">21036333</PMID><DateCompleted><Year>2011</Year><Month>02</Month><Day>15</Day></DateCompleted><DateRevised><Year>2019</Year><Month>12</Month><Day>10</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Print">1878-6146</ISSN><JournalIssue CitedMedium="Internet"><Volume>114</Volume><Issue>11-12</Issue><PubDate><MedlineDate>2010 Nov-Dec</MedlineDate></PubDate></JournalIssue><Title>Fungal biology</Title><ISOAbbreviation>Fungal Biol</ISOAbbreviation></Journal><ArticleTitle>Endophytic fungal diversity in Theobroma cacao (cacao) and T. grandiflorum (cupuaçu) trees and their potential for growth promotion and biocontrol of black-pod disease.</ArticleTitle><Pagination><MedlinePgn>901-10</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1016/j.funbio.2010.08.006</ELocationID><Abstract><AbstractText>The endophytic niches of plants are a rich source of microbes that can directly and indirectly promote plant protection, growth and development. The diversity of culturable endophytic fungi from stems and branches of Theobroma cacao (cacao) and Theobroma grandiflorum (cupuaçu) trees growing in the Amazon region of Brazil was assessed. The collection of fungal endophytic isolates obtained was applied in field experiments to evaluate their potential as biocontrol agents against Phytophthora palmivora, the causal agent of the black-pod rot disease of cacao, one of the most important pathogens in cocoa-producing regions worldwide. The isolated endophytic fungi from 60 traditional, farmer-planted, healthy cacao and 10 cupuaçu plants were cultured in PDA under conditions inducing sporulation. Isolates were classified based upon the morphological characteristics of their cultures and reproductive structures. Spore suspensions from a total of 103 isolates that could be classified at least up to genus level were tested against P. palmivora in pods attached to cacao trees in the field. Results indicated that ∼70% of isolates showed biocontrol effects to a certain extent, suggesting that culturable endophytic fungal biodiversity in this system is of a mostly mutualistic type of interaction with the host. Eight isolates from genera Trichoderma (reference isolate), Pestalotiopsis, Curvularia, Tolypocladium and Fusarium showed the highest level of activity against the pathogen, and were further characterized. All demonstrated their endophytic nature by colonizing axenic cacao plantlets, and confirmed their biocontrol activity on attached pods trials by showing significant decrease in disease severity in relation to the positive control. None, however, showed detectable growth-promotion effects. Aspects related to endophytic biodiversity and host-pathogen-endophyte interactions in the environment of this study were discussed on the context of developing sustainable strategies for biological control of black-pod rot of cacao.</AbstractText><CopyrightInformation>Copyright © 2010 The British Mycological Society. Published by Elsevier Ltd. All rights reserved.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Hanada</LastName><ForeName>Rogério Eiji</ForeName><Initials>RE</Initials><AffiliationInfo><Affiliation>Instituto Nacional de Pesquisas da Amazônia (INPA/CPPF), Av. André Araujo, 2936, Petrópolis, Cx. Postal 478, 69083-000 Manaus-AM, Brazil.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Pomella</LastName><ForeName>Alan William V</ForeName><Initials>AW</Initials></Author><Author ValidYN="Y"><LastName>Costa</LastName><ForeName>Heron Salazar</ForeName><Initials>HS</Initials></Author><Author ValidYN="Y"><LastName>Bezerra</LastName><ForeName>José Luiz</ForeName><Initials>JL</Initials></Author><Author ValidYN="Y"><LastName>Loguercio</LastName><ForeName>Leandro L</ForeName><Initials>LL</Initials></Author><Author ValidYN="Y"><LastName>Pereira</LastName><ForeName>José O</ForeName><Initials>JO</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D023362">Evaluation Study</PublicationType><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2010</Year><Month>09</Month><Day>15</Day></ArticleDate></Article><MedlineJournalInfo><Country>Netherlands</Country><MedlineTA>Fungal Biol</MedlineTA><NlmUniqueID>101524465</NlmUniqueID></MedlineJournalInfo><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000898" MajorTopicYN="N">Antibiosis</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D044822" MajorTopicYN="Y">Biodiversity</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D002099" MajorTopicYN="N">Cacao</DescriptorName><QualifierName UI="Q000382" MajorTopicYN="N">microbiology</QualifierName><QualifierName UI="Q000469" MajorTopicYN="Y">parasitology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D005658" MajorTopicYN="N">Fungi</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000302" MajorTopicYN="N">isolation & purification</QualifierName><QualifierName UI="Q000502" MajorTopicYN="Y">physiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010572" MajorTopicYN="N">Pest Control, Biological</DescriptorName><QualifierName UI="Q000379" MajorTopicYN="Y">methods</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010838" MajorTopicYN="N">Phytophthora</DescriptorName><QualifierName UI="Q000502" MajorTopicYN="Y">physiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010935" MajorTopicYN="N">Plant Diseases</DescriptorName><QualifierName UI="Q000469" MajorTopicYN="Y">parasitology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D013559" MajorTopicYN="N">Symbiosis</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D014197" MajorTopicYN="N">Trees</DescriptorName><QualifierName UI="Q000382" MajorTopicYN="N">microbiology</QualifierName><QualifierName UI="Q000469" MajorTopicYN="Y">parasitology</QualifierName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2010</Year><Month>06</Month><Day>30</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2010</Year><Month>08</Month><Day>24</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2010</Year><Month>11</Month><Day>2</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2010</Year><Month>11</Month><Day>3</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2011</Year><Month>2</Month><Day>16</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">21036333</ArticleId><ArticleId IdType="pii">S1878-6146(10)00136-4</ArticleId><ArticleId IdType="doi">10.1016/j.funbio.2010.08.006</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>Brésil</li></country><region><li>Amazonas (Brésil)</li></region></list><tree><noCountry><name sortKey="Bezerra, Jose Luiz" sort="Bezerra, Jose Luiz" uniqKey="Bezerra J" first="José Luiz" last="Bezerra">José Luiz Bezerra</name><name sortKey="Costa, Heron Salazar" sort="Costa, Heron Salazar" uniqKey="Costa H" first="Heron Salazar" last="Costa">Heron Salazar Costa</name><name sortKey="Loguercio, Leandro L" sort="Loguercio, Leandro L" uniqKey="Loguercio L" first="Leandro L" last="Loguercio">Leandro L. Loguercio</name><name sortKey="Pereira, Jose O" sort="Pereira, Jose O" uniqKey="Pereira J" first="José O" last="Pereira">José O. Pereira</name><name sortKey="Pomella, Alan William V" sort="Pomella, Alan William V" uniqKey="Pomella A" first="Alan William V" last="Pomella">Alan William V. Pomella</name></noCountry><country name="Brésil"><region name="Amazonas (Brésil)"><name sortKey="Hanada, Rogerio Eiji" sort="Hanada, Rogerio Eiji" uniqKey="Hanada R" first="Rogério Eiji" last="Hanada">Rogério Eiji Hanada</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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